CD63 Protein, Mouse, Recombinant

• Background:

  The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 63 (CD63) is a member of the CD family and the transmembrane 4 superfamily, also known as the tetraspanin family. CD63 is a cell surface glycoprotein characterized by the presence of four hydrophobic domains. CD63 had functions in mediating signal transduction processes and then regulate a variety of cellular processes such as cell proliferation, activation and motility. It has been reported that CD63 protein associated with tumor progression and served as a blood platelet activation marker and the deficiency of this protein may be associated with Hermansky-Pudlak syndrome.

• Synonyms:

  C75951 Protein, Mouse; ME491 Protein, Mouse; Tspan30 Protein, Mouse

• Gene Name:

  CD63 molecule

• Expression System:

  HEK293 Cells

• Tag:

  No tag

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Diagnostic proteins, CD Molecule proteins, Enzyme Proteins

• Molecular Weight:

  The recombinant rat Cd63 consists 103 amino acids and predicts a molecular mass of 11.6 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CD63

• Species:

  Mouse

• Protein ID:

  NP_031679.1

• Overview:

  A DNA sequence encoding the rat Cd63 (NP_031679.1) (Ala103-Ile203) was expressed with two additional amino acids (Gly & Pro) at the N-terminus.

• AA Sequence:

  Ala103-Ile203

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_031679.1