Mocs3 (NM_001160330) Mouse Recombinant Protein

• Background:

  Plays a central role in 2-thiolation of mcm (5) S (2) U at tRNA wobble positions of cytosolic tRNA (Lys), tRNA (Glu) and tRNA (Gln) . Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  1700020H17Rik; Uba4

• Gene ID:

  69372

• UniProt:

  A2BDX3

• Accession Number mRNA:

  NM_001160330

• Chromosomal Location:

  2 H3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  49.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Mocs3

• Species:

  Mouse

• Protein ID:

  NP_001153802

• Overview:

  Purified recombinant protein of Mouse molybdenum cofactor synthesis 3 (Mocs3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/69372

• Uniprot URL:

  https://www.uniprot.org/uniprot/A2BDX3

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001160330

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001153802