Ulk3 (NM_027895) Mouse Recombinant Protein

• Background:

  Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3) . Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  1200015E14Rik

• Gene ID:

  71742

• UniProt:

  Q3U3Q1

• Accession Number mRNA:

  NM_027895

• Chromosomal Location:

  9 B

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  53.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ulk3

• Species:

  Mouse

• Protein ID:

  NP_082171

• Overview:

  Purified recombinant protein of Mouse unc-51-like kinase 3 (Ulk3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/71742

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q3U3Q1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_027895

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_082171