Pitrm1 (NM_145131) Mouse Recombinant Protein

• Background:

  Metalloendopeptidase of the mitochondrial matrix that functions in peptide cleavage and degradation rather than in protein processing. Has an ATP-independent activity. Specifically cleaves peptides in the range of 5 to 65 residues. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Degrades the transit peptides of mitochondrial proteins after their cleavage. Also degrades other unstructured peptides. It is also able to degrade amyloid-beta protein 40, one of the peptides produced by APP processing, when it accumulates in mitochondrion. It is a highly efficient protease, at least toward amyloid-beta protein 40. Cleaves that peptide at a specific position and is probably not processive, releasing digested peptides intermediates that can be further cleaved subsequently.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  2310012C15Rik; AA410010; mKIAA1104; MP-1; MP1; Ntup1; PreP

• Gene ID:

  69617

• UniProt:

  Q8K411

• Accession Number mRNA:

  NM_145131

• Chromosomal Location:

  13 A1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  117.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Pitrm1

• Species:

  Mouse

• Protein ID:

  NP_660113

• Overview:

  Purified recombinant protein of Mouse pitrilysin metallepetidase 1 (Pitrm1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/69617

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8K411

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_145131

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_660113