Rpe65 (NM_029987) Mouse Recombinant Protein

• Background:

  Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore (PubMed:15765048, PubMed:9843205, PubMed:23407971, PubMed:28500718) . Essential for the production of 11-cis retinal for both rod and cone photoreceptors (PubMed:17251447) . Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  65kDa; A930029L06Rik; LCA2; Mord1; rd12; RP20

• Gene ID:

  19892

• UniProt:

  Q91ZQ5

• Accession Number mRNA:

  NM_029987

• Chromosomal Location:

  3 82.52 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  61.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Rpe65

• Species:

  Mouse

• Protein ID:

  NP_084263

• Overview:

  Purified recombinant protein of Mouse retinal pigment epithelium 65 (Rpe65), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/19892

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q91ZQ5

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_029987

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_084263