Xrcc2 (NM_020570) Mouse Recombinant Protein

• Background:

  Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  4921524O04Rik; 8030409M04Rik; RAD51; RecA

• Gene ID:

  57434

• UniProt:

  Q9CX47

• Accession Number mRNA:

  NM_020570

• Chromosomal Location:

  5 B1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  31.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Xrcc2

• Species:

  Mouse

• Protein ID:

  NP_065595

• Overview:

  Purified recombinant protein of Mouse X-ray repair complementing defective repair in Chinese hamster cells 2 (Xrcc2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/57434

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9CX47

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_020570

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_065595