Smad7 (NM_001042660) Mouse Recombinant Protein

• Background:

  Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Madh7

• Gene ID:

  17131

• UniProt:

  O35253, B2RPW6

• Accession Number mRNA:

  NM_001042660

• Chromosomal Location:

  18 E3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  46.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Smad7

• Species:

  Mouse

• Protein ID:

  NP_001036125

• Overview:

  Purified recombinant protein of Mouse SMAD family member 7 (Smad7), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/17131

• Uniprot URL:

  https://www.uniprot.org/uniprot/O35253

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001042660

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001036125