Ern1 (NM_023913) Mouse Recombinant Protein

• Background:

  Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11850408, PubMed:25164867) . In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:25164867) . The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11850408, PubMed:25164867) . The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11850408, PubMed:25164867) . Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  9030414B18Rik; AI225830; C85377; Ire1a; Ire1alpha; Ire1p

• Gene ID:

  78943

• UniProt:

  Q9EQY0

• Accession Number mRNA:

  NM_023913

• Chromosomal Location:

  11 E1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  110.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ern1

• Species:

  Mouse

• Protein ID:

  NP_076402

• Overview:

  Purified recombinant protein of Mouse endoplasmic reticulum (ER) to nucleus signalling 1 (Ern1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/78943

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9EQY0

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_023913

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_076402