Her2 (ERBB2) (NM_004448) Human Recombinant Protein

• Background:

  This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

• Synonyms:

  CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1

• Gene ID:

  2064

• UniProt:

  P04626, X5DNK3

• Accession Number mRNA:

  NM_004448

• Chromosomal Location:

  17q12

• Expression System:

  Sf9

• Tag:

  C-DDK

• Related Pathways:

  Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer

• Field of Research:

  Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris-HCl pH8.0, 100mM glycine, 10% glycerol

• Function:

  Druggable Genome, Protein Kinase, Transmembrane

• Molecular Weight:

  138 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ERBB2

• Species:

  Human

• Protein ID:

  NP_004439

• Overview:

  Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), full length, with C-terminal DDK tag, expressed in sf9 cells.

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/2064

• Uniprot URL:

  https://www.uniprot.org/uniprot/P04626

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_004448

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_004439