TNF alpha (TNF) (NM_000594) Human Recombinant Protein

• Background:

  This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Aug 2020]

• Synonyms:

  DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F

• Gene ID:

  7124

• UniProt:

  P01375, Q5STB3

• Accession Number mRNA:

  NM_000594

• Chromosomal Location:

  6p21.33

• Expression System:

  E. coli

• Tag:

  Tag Free

• Related Pathways:

  Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus

• Field of Research:

  Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus

• Concentration:

  Resuspend the protein to the desired concentration in proper buffer.

• Endotoxin:

  < 0.1 EU per 1 µg of the protein by the LAL

• Purity:

  >95% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  Determined by the cytolysis of L929 cells in the presence of Actinomycin D. ED50 for this effect is typically 0.19-0.27 ng/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from a sterile solution containing 20 mM phosphate buffer, PH7.2.

• Function:

  Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane

• Molecular Weight:

  17.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  TNF

• Species:

  Human

• Protein ID:

  NP_000585

• Overview:

  Recombinant protein of human Tumor Necrosis Factor-a (TNFa) produced in E. coli.

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/7124

• Uniprot URL:

  https://www.uniprot.org/uniprot/P01375

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000594

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000585