TNF-alpha Protein, Human, Recombinant

• Background:

  Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1) . TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

• Synonyms:

  DIF Protein, Human; TNF-alpha Protein, Human; TNFA Protein, Human; TNFSF2 Protein, Human

• Gene Name:

  Tumor necrosis factor

• Expression System:

  E. coli

• Tag:

  No tag

• Field of Research:

  Cancer Drug TargetsNeuroinflammation

• Endotoxin:

  < 5 EU per mg of the protein as determined by the LAL method.

• Purity:

  ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

• Activity:

  Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The EDfor this effect is typically 3-30 pg/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Diagnostic proteins, TNF Superfamily proteins, Immunotherapy proteins, Cell Therapy proteins, Cytokine Proteins, Growth Factor proteins, Biological Target proteins, Biomarker Proteins

• Molecular Weight:

  The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa which is also estimated by SDS-PAGE.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  TNF

• Species:

  Human

• Protein ID:

  NP_000585.2

• Overview:

  A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.

• AA Sequence:

  Val77-Leu233

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_000585.2