FGF10 (NM_004465) Human Recombinant Protein

• Background:

  The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq, Jul 2008]

• Synonyms:

  Fibroblast growth factor 10; keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria

• Gene ID:

  2255

• UniProt:

  O15520

• Accession Number mRNA:

  NM_004465

• Chromosomal Location:

  5p12

• Expression System:

  E. coli

• Tag:

  Tag Free

• Related Pathways:

  MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton

• Field of Research:

  MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton

• Concentration:

  Resuspend the protein to the desired concentration in proper buffer.

• Endotoxin:

  < 0.1 EU per 1 µg of the protein by the LAL

• Purity:

  >95% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  Determined by the dose-dependant stimulation of 4MBr-5 cells. ED50 for this effect is typically 14 - 21 ng/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH7.4.

• Function:

  Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein, Transcription Factors, Transmembrane

• Molecular Weight:

  19.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FGF10

• Species:

  Human

• Protein ID:

  NP_004456

• Overview:

  Recombinant protein of human Fibroblast Growth Factor-10 (FGF10) produced in E. coli.

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/2255

• Uniprot URL:

  https://www.uniprot.org/uniprot/O15520

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_004465

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_004456