Factor VIII (F8) (NM_019863) Human Recombinant Protein

• Background:

  This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]

• Synonyms:

  AHF; DXS1253E; F8B; F8C; FVIII; HEMA

• Gene ID:

  2157

• UniProt:

  P00451

• Accession Number mRNA:

  NM_019863

• Chromosomal Location:

  Xq28

• Expression System:

  E. coli

• Tag:

  N-His

• Related Pathways:

  Complement and coagulation cascades

• Field of Research:

  Complement and coagulation cascades

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris, 8M Urea, pH8.0.

• Function:

  Druggable Genome, Secreted Protein

• Molecular Weight:

  24.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  F8

• Species:

  Human

• Protein ID:

  NP_063916

• Overview:

  Purified recombinant protein of Human coagulation factor VIII, procoagulant component (F8), transcript variant 2, full length, with N-terminal HIS tag, expressed in E. coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/2157

• Uniprot URL:

  https://www.uniprot.org/uniprot/P00451

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_019863

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_063916