IL1-F8 Protein, Human, Recombinant

• Background:

  Interleukin-1 family member 8 (IL1F8) also known as IL36B, is a member of the interleukin 1 (IL-1) cytokine family. IL1F8 may contain a 12-stranded beta-trefoil structure. Until recently, the IL-1 family of cytokines includes four members, with three having pro-inflammatory effects such as IL1F8 and the fourth member being an IL-1 receptor antagonist (IL-1Ra) . IL-1 family members exert their effects through binding to receptors that belong to the IL-1 receptor (IL-1R) family. IL1F8 exerts proinflammatory effects in primary human joint cells. Joint and serum IL-1F8 protein levels did not correlate with inflammation, but they were high in some human serum samples tested, including samples from patients with rheumatoid arthritis. IL1F8 also activated NK-κB.

• Synonyms:

  FIL1 Protein, Human; FIL1- (ETA) Protein, Human; FIL1H Protein, Human; FILI- (ETA) Protein, Human; IL-1F8 Protein, Human; IL-1H2 Protein, Human; IL1-ETA Protein, Human; IL1F8 Protein, Human; IL1H2 Protein, Human; IL36B Protein, Human

• Gene Name:

  Interleukin 36, beta

• UniProt:

  Q9NZH7-2

• Expression System:

  E. coli

• Tag:

  No tag

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 97 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Biological Target proteins, Cytokine Proteins, Growth Factor proteins, Interleukin proteins

• Molecular Weight:

  The recombinant human IL36B consists of 157 amino acids and has a predicted molecular mass of 17.7 kDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IL36B

• Species:

  Human

• Overview:

  A DNA sequence encoding the mature form of human IL36B isofrom 2 (Q9NZH7-2) (Met 1-Glu 157) was expressed.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9NZH7-2

• AA Sequence:

  Met1-Glu157