POLR3F (NM_006466) Human Recombinant Protein

• Background:

  The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB) . Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

• Synonyms:

  C34; RPC6; RPC39

• Gene ID:

  10621

• UniProt:

  Q9H1D9

• Accession Number mRNA:

  NM_006466

• Chromosomal Location:

  20p11.23

• Expression System:

  E. coli

• Tag:

  N-GST and C-His

• Related Pathways:

  Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

• Field of Research:

  Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.

• Function:

  Transcription Factors

• Molecular Weight:

  61.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  POLR3F

• Species:

  Human

• Protein ID:

  NP_006457

• Overview:

  Purified recombinant protein of Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/10621

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9H1D9

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_006466

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_006457