Isocitrate dehydrogenase (IDH1) Mouse Monoclonal Antibody [Clone ID: LBI2H9]

• Background:

  Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+) -dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+) -dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

• Synonyms:

  HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD

• Gene Name:

  Isocitrate dehydrogenase (NADP (+) ) 1

• Gene ID:

  3417

• UniProt:

  O75874

• Conjugation:

  Unconjugated

• Related Pathways:

  Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways

• Field of Research:

  Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways

• Concentration:

  1 mg/ml

• Dilution:

  WB 1:2000, IHC 1:50, IF 1:100

• Form:

  PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

• Buffer:

  PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

• Storage Conditions:

  Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

• Symbol:

  IDH1

• Overview:

  Anti-IDH1 (Isocitrate Dehydrogenase 1) mouse monoclonal antibody, clone LBI2H9

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/?term=3417

• Uniprot URL:

  https://www.uniprot.org/uniprot/O75874