Isocitrate dehydrogenase (IDH1) (NM_005896) Human Recombinant Protein

• Background:

  Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+) -dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+) -dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]

• Synonyms:

  HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD

• Gene ID:

  3417

• UniProt:

  O75874, A0A024R3Y6

• Accession Number mRNA:

  NM_005896

• Chromosomal Location:

  2q34

• Expression System:

  Sf9

• Tag:

  C-DDK

• Related Pathways:

  Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways

• Field of Research:

  Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris-HCl pH8.0, 150mM NaCl, 10%glycerol

• Molecular Weight:

  47 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IDH1

• Species:

  Human

• Protein ID:

  NP_005887

• Overview:

  Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant (R132C) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/3417

• Uniprot URL:

  https://www.uniprot.org/uniprot/O75874

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_005896

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_005887