BAFF/BLyS Protein, Canine, Recombinant (hFc Tag)

• Background:

  B lymphocyte stimulator (BLyS), also known as TNFSF13B, CD257 and BAFF, is a single-pass type II membrane protein, which belongs to the tumor necrosis factor family. BAFF is abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. BAFF is a cytokine and serves as a ligand for receptors TNFRSF13B (TACI), TNFRSF17 (BCMA), and TNFRSF13C (BAFFR) . These receptors are a prominent factor in B cell differentiation, homeostasis, and selection. BLyS levels affect survival signals and selective apoptosis of autoantibody-producing B cells. Thus, it acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells. Overexpression of BLyS in mice can lead to clinical and serological features of systemic lupus erythematosus (SLE) and Sj gren's syndrome (SS) . BLyS is an attractive therapeutic target in human rheumatic diseases. The ability of BLyS to regulate both the size and repertoire of the peripheral B cell compartment raises the possibility that BLyS and antagonists thereof may form the basis of a therapeutic trichotomy. As an agonist, BLyS protein may enhance humoral immunity in congenital or acquired immunodeficiencies such as those resulting from viral infection or cancer therapy.

• Gene Name:

  Tumor necrosis factor (ligand) superfamily, member 13b

• Expression System:

  HEK293 Cells

• Tag:

  N-hFc

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measured in a cell proliferation assay using mouse splenocytes. The EDfor this effect is typically 1-5 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  TNF Superfamily proteins, CD Molecule proteins, Immunotherapy proteins, Cytokine Proteins, Growth Factor proteins, Biological Target proteins

• Molecular Weight:

  The recombinant canine TNFSF13B consists 412 amino acids and predicts a molecular mass of 45.5 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  TNFSF13B

• Species:

  Canine

• Protein ID:

  NP_001155182.1

• Overview:

  A DNA sequence encoding the canine TNFSF13B (NP_001155182.1) (Ala143-Leu292) was expressed with the Fc region of human IgG1 at the N-terminus.

• AA Sequence:

  Ala143-Leu292

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_001155182.1