BMP-2 Protein, Danio rerio (zebrafish), Recombinant

• Background:

  BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.

• Gene Name:

  Bone morphogenetic protein 2

• UniProt:

  B3DI86

• Expression System:

  E. coli

• Tag:

  No tag

• Field of Research:

  Early Mesodermal Lineage Markers

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The EDfor this effect is typically 0.5-3 μg/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 30mM HAC, pH 3.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  TGF beta Family proteins, Cytokine Proteins, Growth Factor proteins, Cell Therapy proteins, Protease proteins, Biological Target proteins, Enzyme Proteins

• Molecular Weight:

  The recombinant zebrafish BMP2A consists of 116 amino acids and has a calculated molecular mass of 13.2 kDa. It migrates as an 13 KDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  BMP2

• Species:

  Danio rerio (zebrafish)

• Overview:

  A DNA sequence encoding the zebrafish BMP2A (B3DI86) (Gln272-Arg386) was expressed and purified with an initial Met.

• Uniprot URL:

  https://www.uniprot.org/uniprot/B3DI86

• AA Sequence:

  Gln272-Arg386