C. Perfringens Neuraminidase Protein, C.perfringens, Recombinant (His Tag)

• Background:

  Clostridium perfringens / C. perfringens (formerly known as C. welchii) is a Gram-positive, rod-shaped, anaerobic, spore-forming bacterium of the genus Clostridium. C. perfringens is ubiquitous in nature and can be found as a normal component of decaying vegetation, marine sediment, the intestinal tract of humans and other vertebrates, insects, and soil. C. perfringens is commonly encountered in infections as a benign component of the normal flora. In this case, its role in disease is minor. Infections due to C. perfringens show evidence of tissue necrosis, bacteremia, emphysematous cholecystitis, and gas gangrene, which is also known as clostridial myonecrosis. NA, also called sialidases, specifically catalyze the hydrolysis removal of terminal sialic acid residues from viral and cellular glycoconjugates. C. Perfringens neuraminidase catalyzes the hydrolysis of alpha- (2->3) -, alpha- (2->6) -, glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates, but has little activity against the α2-8 glycosidic linkages. The function of the neuraminidase is to release sialic acids for use as carbon and energy sources for the non-pathogenic bacterium, while in pathogenic microorganisms, sialidases have been suggested to be pathogenic factors

• Synonyms:

  NA Protein, C.perfringens

• Expression System:

  E. coli

• Tag:

  N-His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 97 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to cleave a fluorogenic substrate, 2'- (4-Methylumbelliferyl) -α-D-N-acetylneuraminic acid. The specific activity is 150,000 pmoles/min/μg (Activity of this protein is much better than the world's famous brand) as measured under the described conditions.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant C. Perfringens neuraminidase consisting of 388 amino acids and has a calculated molecular mass of 43.6 kDa. It migrates as an approximately 40 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  C. Perfringens NA

• Species:

  C.perfringens

• Protein ID:

  P10481.1

• Overview:

  A DNA sequence encoding the C. Perfringens neuraminidase (P10481.1) (Cys 2-Gln 382) was expressed, with a N-terminal polyhistidine tag.

• AA Sequence:

  Cys2-Gln382

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/P10481.1