NKG2A Protein, Cynomolgus, Rhesus, Recombinant (hFc Tag)

• Background:

  NKG2, also known as NKG2A (CD159A), is a member of the killer cell lectin-like receptor family. This family is a group of transmembrane proteins preferentially expressed in NK cells. Members of this family are characterized by the type II membrane orientation and the presence of a C-type lectin domain. NKG2 contains 1 C-type lectin domain and forms a complex with another family member, KLRD1/CD94. It is expressed only in NK-cells, but not in T-cells or B-cells. It has been shown that NKG2 represents a family of related cDNA clones, designated NKG2A, NKG2B, NKG2C, and NKG2D, which encode type 2 integral membrane proteins (extracellular C-terminus) containing a C-type lectin domain. Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NKG2 functions as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.

• Gene Name:

  Killer cell lectin-like receptor subfamily C, member 1

• UniProt:

  Q9MZJ2

• Expression System:

  HEK293 Cells

• Tag:

  N-hFc

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  CD Molecule proteins, Receptor Proteins, Immune Checkpoint Proteins, Biological Target proteins

• Molecular Weight:

  The recombinant cynomolgus / rhesus KLRC1 is a disulfide-linked homodimer. The reduced monomer comprises 400 amino acids and has a calculated molecular mass of 44.4 KDa.The apparent molecular mass of the protein is approximately 47-55 KDa respectively in SDS-PAGE.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  KLRC1

• Species:

  Cynomolgus, Rhesus

• Protein ID:

  NP_001271106.1

• Overview:

  A DNA sequence encoding the cynomolgus / rhesus KLRC1 (NP_001271106.1/Q9MZJ2) (Pro94-Leu233) was expressed with the Fc region of human IgG1 at the N-terminus. Cynomolgus and Rhesus KLRC1 sequences are identical.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9MZJ2

• AA Sequence:

  Pro94-Leu233

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_001271106.1