HGF Protein, Cynomolgus, Recombinant

• Background:

  Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain, and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. The hepatocyte growth factor is secreted by mesenchymal cells as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility and matrix invasion give it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

• Gene Name:

  Hepatocyte growth factor (hepapoietin A; scatter factor)

• UniProt:

  C9E9X5

• Expression System:

  HEK293 Cells

• Tag:

  No tag

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  1. Immobilized HGF Protein, Cynomolgus, Recombinant (Cat:PK54072M0) at 2 μg/mL (100 μL/well) can bind c-MET Protein, Cynomolgus, Rhesus, Recombinant (His Tag) (Cat:PK54241M2), the ECis 60-240 ng/mL. 2. Measured by its ability to inhibit TGF 1 activity on Mv-1-lu mink lung epithelial cells. The EDfor this effect is typically 2-10 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cancer proteins, Cytokine Proteins, Biological Target proteins, Diagnostic proteins, Cell Therapy proteins

• Molecular Weight:

  The recombinant Cynomolgus HGF consists of 697 amino acids and has a calculated molecular mass of 79.6 KDa. It migrates as 89,58,34 and 32 KDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  HGF

• Species:

  Cynomolgus

• Overview:

  A DNA sequence encoding the cynomolgus HGF (C9E9X5) (Met1-Ser728) was expressed.

• Uniprot URL:

  https://www.uniprot.org/uniprot/C9E9X5

• AA Sequence:

  Met1-Ser728