Interferon alpha-B Protein, Cynomolgus, Recombinant (His Tag)

• Background:

  Interferon alpha-B, also known as IFNA8, belongs to the alpha/beta interferon family. Interferons are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites, or tumor cells. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They also allow for communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. Interferons also activate immune cells, such as natural killer cells and macrophages. They increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes. They also increase the ability of uninfected host cells to resist new infections by virus. Certain symptoms, such as aching muscles and fever, are related to the production of IFNs during infection. Produced by macrophages, IFN-alpha has antiviral activities.

• Gene Name:

  Interferon, alpha 8

• UniProt:

  G7PS89

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus.The EDfor this effect is typically 1-5 pg/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Biological Target proteins, Cytokine Proteins

• Molecular Weight:

  The recombinant cynomolgus IFNA8 comprises 177 amino acids and has a calculated molecular mass of 21 KDa. The apparent molecular mass of it is approximately 33 KDa respectively in SDS-PAGE.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IFNA8

• Species:

  Cynomolgus

• Overview:

  A DNA sequence encoding the cynomolgus IFNA8 (G7PS89) (Met1-Glu189) was expressed with a polyhistidine tag at the C-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/G7PS89

• AA Sequence:

  Met1-Glu189