CD16/FCGR3 Protein, Rhesus, Recombinant (His Tag)

• Background:

  Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR) . FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16) . CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

• Gene Name:

  Fc receptor, IgG, low affinity III

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Fc Receptors

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured by its binding ability in a functional ELISA.Immobilized Rhesus FCGR3-His (cat:PK54091M2) at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc) (Cat:PH52028M0) . The ECof human IgG1 (Fc) (Cat:PH52028M0) is 0.35-0.81 μg/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  CD Molecule proteins, Receptor Proteins, Fc receptor proteins

• Molecular Weight:

  The recombinant rhesus CD16 consists of 201 amino acids and has a calculated molecular mass of 22.7 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FCGR3

• Species:

  Rhesus

• Protein ID:

  NP_001258584.1

• Overview:

  A DNA sequence encoding the rhesus CD16 (NP_001258584.1) extracellular domain (Met 1-Gly 206) was fused with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Gly206

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_001258584.1