CD16/FCGR3 Protein, Rhesus, Recombinant (His & AVI Tag), Biotinylated

• Background:

  Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR) . FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16) . CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

• Gene Name:

  Fc receptor, IgG, low affinity III

• Expression System:

  HEK293 Cells

• Tag:

  C-AVI & His

• Field of Research:

  Fc Receptors

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 92 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to bind human IgG1 in a functional ELISA.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Fc receptor proteins, Biotinylated proteins, CD Molecule proteins, Receptor Proteins

• Molecular Weight:

  The recombinant rhesus CD16 consists of 223 amino acids and has a calculated molecular mass of 25.3 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FCGR3

• Species:

  Rhesus

• Protein ID:

  NP_001258584.1

• Overview:

  A DNA sequence encoding the rhesus CD16 (NP_001258584.1) extracellular domain (Met 1- Gln 208) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin–Protein ligase (BirA enzyme) which is co-expressed.

• AA Sequence:

  Met1-Gln208

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_001258584.1