CD86 Protein, Cynomolgus, Rhesus, Recombinant (hFc Tag)

• Background:

  CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

• Gene Name:

  CD86 antigen

• UniProt:

  Q9BDM4

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  B7 Family proteins, CD Molecule proteins, Cancer proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins, Diagnostic proteins

• Molecular Weight:

  The recombinant cynomolgus / rhesus CD86 is a disulfide-linked homodimer. The reduced monomer comprises 461 amino acids and has a calculated molecular mass of 52.2 KDa.The apparent molecular mass of the protein is approximately 76-86 and 34 KDa respectively in SDS-PAGE.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CD86

• Species:

  Cynomolgus, Rhesus

• Overview:

  A DNA sequence encoding the cynomolgus / rhesus CD86 (XP_005548057.1/Q9BDM4) (Met1-His239) was expressed with the Fc region of human IgG1 at the C-terminus. Cynomolgus and Rhesus CD86 sequences are identical.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9BDM4

• AA Sequence:

  Met1-His239