IFNGR1 Protein, Rat, Recombinant (hFc Tag)

• Background:

  The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alters the behavior of the cell. Some CD proteins do not take part in the cell signal process but have other functions such as cell adhesion. CD119 (cluster of differentiation 119), also known as IFNGR1 (interferon-gamma receptor 1), is part of the heterodimeric gamma interferon receptor which consists of IFNGR1 (CD119) and IFNGR2. The IFNGR1 gene encodes the ligand-binding chain (alpha) of the interferon receptor while the IFNGR gene encodes the non-ligand binding partner. The ability of the interferon-γ was achieved through binding to the interferon receptor CD119. After binding, the products of activated T-lymphocytes interferon-γ exerts antiviral activity, growth inhibitory effect, and several immune- regulatory activities on a variety of cell types.

• Gene Name:

  Interferon gamma receptor 1

• UniProt:

  Q6P6T3

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  1. Measured by its binding ability in a functional ELISA. Immobilized Rat IFNGR1 hFc (Cat:PR53778M4) at 2 μg/ml (100 μl/well) can bind Rat Interferon Gamma hFc (Cat:PR53614M4), Biotinylated the ECof Rat Interferon Gamma hFc, Biotinylated is 60-300 ng/mL. 2. Measured by its ability to inhibit rat IFNγ-mediated protection of L929 cells infected with vesicular stomatitisvirus (VSV) . The EDfor this effect is typically 0.5-4 ng/mL in the presence of 10 ng/mL of recombinant rat IFN-gamma.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Interleukin proteins, CD Molecule proteins, Receptor Proteins

• Molecular Weight:

  The recombinant rat IFNGR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 466 amino acids and has a predicted molecular mass of 52.4 kDa. The apparent molecular mass of the protein is approximately 117 and 65-80 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IFNGR1

• Species:

  Rat

• Overview:

  A DNA sequence encoding the rat IFNGR (Q6P6T3) (Met1-Ser241) was expressed with the Fc region of human IgG1 at the C-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q6P6T3

• AA Sequence:

  Met1-Ser241