MCP-1/CCL2 Protein, Mouse, Recombinant (His Tag), Biotinylated

• Background:

  Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.

• Synonyms:

  AI323594 Protein, Mouse; HC11 Protein, Mouse; JE Protein, Mouse; MCAF Protein, Mouse; MCP-1 Protein, Mouse; MCP1 Protein, Mouse; Scya2 Protein, Mouse; Sigje Protein, Mouse; SMC-CF Protein, Mouse

• Gene Name:

  Chemokine (C-C motif) ligand 2

• Expression System:

  Baculovirus-Insect cells

• Tag:

  C-His

• Field of Research:

  Neuroinflammation

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Biotinylated proteins, Biomarker Proteins, Cell Therapy proteins, Growth Factor proteins, Biological Target proteins, Immunotherapy proteins, Diagnostic proteins, Cytokine Proteins

• Molecular Weight:

  The mouse CCL2 consists of 135 amino acids and predicts a molecular mass of 15.2 kDa. The apparent molecular mass of the protein is approximately 27 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CCL2

• Species:

  Mouse

• Protein ID:

  NP_035463.1

• Overview:

  A DNA sequence encoding the mouse CCL2 (NP_035463.1) (Met1-Asn148) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.

• AA Sequence:

  Met1-Asn148

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_035463.1