IL12B Protein, Mouse, Recombinant

• Background:

  Subunit beta of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) (IL12B) is a subunit of human interleukin 12. IL12B/IL-12B is a cytokine that acts on T and natural killer cells and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor-like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. IL12B/IL-12B is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine is important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. IL12B/IL-12B associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine that functions in innate and adaptive immunity.

• Synonyms:

  Il-12b Protein, Mouse; Il-12p40 Protein, Mouse; Il12p40 Protein, Mouse; p40 Protein, Mouse

• Gene Name:

  Interleukin 12B

• UniProt:

  P43432

• Expression System:

  HEK293 Cells

• Tag:

  No tag

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to bind Human IL12RB1 His (Cat:PH52590M2), Biotinylated in a functional ELISA.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Immunotherapy proteins, Cell Therapy proteins, Biological Target proteins, Cytokine Proteins, Growth Factor proteins, Interleukin proteins

• Molecular Weight:

  The recombinant mouse IL12B consists of 320 amino acids and has a calculated molecular mass of 36.6 kDa. The recombinant protein migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IL12B

• Species:

  Mouse

• Overview:

  A DNA sequence encoding the mouse IL12B (P43432) (Met 1-Ser 335) was expressed with six amino acids (LEVLFQ) at the C-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P43432

• AA Sequence:

  Met1-Ser335