IL12B (NM_002187) Human Recombinant Protein

• Background:

  This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]

• Synonyms:

  CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF; NKSF2

• Gene ID:

  3593

• UniProt:

  P29460

• Accession Number mRNA:

  NM_002187

• Chromosomal Location:

  5q33.3

• Expression System:

  Sf9

• Tag:

  C-DDK

• Related Pathways:

  Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus

• Field of Research:

  Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris-HCl pH8.0, 150mM NaCl, 20% glycerol

• Function:

  Druggable Genome, Secreted Protein, Transmembrane

• Molecular Weight:

  37 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IL12B

• Species:

  Human

• Protein ID:

  NP_002178

• Overview:

  Recombinant protein of human interleukin 12B (natural killer cell stimulatory factor 2, p40) (IL12B), full length, with C-terminal DDK, expressed in sf9 cells

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/3593

• Uniprot URL:

  https://www.uniprot.org/uniprot/P29460

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_002187

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_002178