TGF beta 2 Protein, Mouse, Recombinant (His Tag)

• Background:

  TGF beta 2 (Transforming growth factor beta 2), an extracellular glycosylated protein, which belongs to the TGF-beta family. TGF-beta regulates key mechanisms of tumor development, namely immunosuppression, metastasis, angiogenesis, and proliferation. TGF beta 2 suppression is a promising therapeutic approach for malignant tumor therapy. The signaling pathway of TGF beta 2/Smad plays an important role in the pathological process in posterior capsule opacification (PCO) after cataract surgery. Silencing Smad2 and Smad3 efficiently blocked the effect of TGF beta 2 on cell proliferation, migration, and extracellular matrix production. TGF beta 2 activation of MEKK3/ERK1/2/5 signaling modulates Has2 expression and hyaluronan (HA) production leading to the induction of epithelial to mesenchymal transformation (EMT) events. Besides, the upregulation of the TGF beta 2 level is a common pathological feature of Alzheimer's disease (AD) brains and suggests that it may be closely linked to the development of neuronal death related to AD.

• Synonyms:

  BB105277 Protein, Mouse; TGF-beta 2 Protein, Mouse; Tgf-beta2 Protein, Mouse; Tgfb-2 Protein, Mouse; TGFB2 Protein, Mouse

• Gene Name:

  Transforming growth factor beta 2

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Early Mesodermal Lineage Markers

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Growth Factor proteins, Biological Target proteins, Diagnostic proteins, Cell Therapy proteins, TGF beta Family proteins, Biomarker Proteins

• Molecular Weight:

  The recombinant mouse Tgfb2 consists 406 amino acids and predicts a molecular mass of 46.9 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  TGFB2

• Species:

  Mouse

• Protein ID:

  NP_033393.2

• Overview:

  A DNA sequence encoding the mouse Tgfb2 (NP_033393.2) (Met1-Ser414) was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Ser414

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_033393.2