CAMKIV Protein, Mouse, Recombinant (His & GST Tag)

• Background:

  Ca/ calmodulin-dependent protein kinase 4 (CAMKIV) belongs to the serine/threonine protein kinase family, and to the Ca/calmodulin-dependent protein kinase subfamily which is widely recognized as an essential enzyme implicated in the phophoinositide amplification cascade. Ca/calmodulin dependent protein kinase (CAMK) can be activated by the introcellular increased Caand then apt to combine with the target protein. Ca/ calmodulin-dependent protein kinase 4 (CAMKIV) is a multifunctional CaM-dependent kinase protein with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. All of the isforms of this family, including myosin light chain kinase, phosphorylase kinase, CaMK1, CaMKIII and CaMKIV have EF-hand structure.

• Synonyms:

  A430110E23Rik Protein, Mouse; AI666733 Protein, Mouse; CaMKIV Protein, Mouse; CaMKIV/Gr Protein, Mouse; D18Bwg0362e Protein, Mouse

• Gene Name:

  Calcium/calmodulin-dependent protein kinase IV

• UniProt:

  P08414

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-GST & His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 94 % as determined by SDS-PAGE.

• Activity:

  No Kinase Activity

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins, Kinase proteins

• Molecular Weight:

  The secreted recombinant mouse CAMK4/GST chimera consists of 706 amino acids and has a calculated molecular mass of 80.4 kDa. The recombinant protein migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CAMK4

• Species:

  Mouse

• Overview:

  A DNA sequence encoding the mouse CAMK4 (P08414) (Met1-Tyr469) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P08414

• AA Sequence:

  Met1-Tyr469