Recombinant Mouse CAMK4/CaMKIV Protein

• Background:

  Ca2+/ calmodulin-dependent protein kinase 4 (CAMKIV) belongs to the serine/threonine protein kinase family, and to the Ca2+/calmodulin-dependent protein kinase subfamily which is widely recognized as an essential enzyme implicated in the phophoinositide amplification cascade. Ca2+/calmodulin dependent protein kinase (CAMK) can be activated by the introcellular increased Ca2+ and then apt to combine with the target protein. Ca2+/ calmodulin-dependent protein kinase 4 (CAMKIV) is a multifunctional CaM-dependent kinase protein with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. All of the isforms of this family, including myosin light chain kinase, phosphorylase kinase, CaMK1, CaMKIII and CaMKIV have EF-hand structure.

• Synonyms:

  A430110E23Rik; AI666733; CaMKIV; CaMKIV/Gr; D18Bwg0362e

• UniProt:

  P08414

• Accession Number:

  P08414

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  None

• Sequence:

  Met1-Tyr469

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Kinase activity untested

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  52.7 kDa

• Observed Molecular Weight:

  55 kDa

• Formulation:

  Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse