Ube2f (NM_026454) Mouse Recombinant Protein

• Background:

  Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  2510010F15Rik; AI851109

• Gene ID:

  67921

• UniProt:

  Q9CY34

• Accession Number mRNA:

  NM_026454

• Chromosomal Location:

  1 D

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  21.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ube2f

• Species:

  Mouse

• Protein ID:

  NP_080730

• Overview:

  Purified recombinant protein of Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/67921

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9CY34

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_026454

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_080730