ICOS Protein, Mouse, Recombinant (His Tag)

• Background:

  Inducible costimulator (ICOS), also called AILIM (Activation-Inducible Lymphocyte Immunomediatory Molecule) is a cell-surface receptor and belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4, and PD-1. The interaction of B7-H2/ICOS plays a critical role in Th cell differentiation, T B cell interactions which are essential for the germinal center formation, and humoral immune responses, and as well as the production of cytokine IL-4. Also, ICOS is more potent in the induction of IL-10 production, a cytokine important for the suppressive function of T regulatory cells. The B7-1/B7-2--CD28/CTLA-4 and ICOS-B7RP-1 pathway provide key second signals that can regulate the activation, inhibition, and fine-tuning of T-lymphocyte responses. ICOS stimulates both Th1 and Th2 cytokine production but may have a preferential role in Th2 cell development. Moreover, The B7-1/B7-2-CD28/CTLA-4 and ICOS-B7RP-1 pathway has been suggested as being involved in the development of airway inflammation and airway hyperresponsiveness.

• Synonyms:

  AILIM Protein, Mouse; CCLP Protein, Mouse; CRP-1 Protein, Mouse; H4 Protein, Mouse; Ly115 Protein, Mouse

• Gene Name:

  Inducible T-cell co-stimulator

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measured by its binding ability in a functional ELISA. Immobilized Rat ICOS ligand hFc (Cat: PR53598M4) at 2 μg/mL (100 μL/well) can bind Mouse ICOS His (Cat:PM52864M2), the ECof Mouse ICOS His (Cat:PM52864M2) is 150-800 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant mouse ICOS consists of 121 amino acids and predicts a molecular mass of 13.9 kDa. As a result of glycosylation, it migrates as an approximately 22.7 and 19.7 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ICOS

• Species:

  Mouse

• Protein ID:

  NP_059508.2

• Overview:

  A DNA sequence encoding the mouse ICOS (NP_034643.2) (Met1-Gln135) was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Gln135

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_059508.2