DDR1 Protein, Mouse, Recombinant (His Tag)

• Background:

  Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.

• Synonyms:

  6030432F18 Protein, Mouse; AI323681 Protein, Mouse; Cak Protein, Mouse; CD167a Protein, Mouse; Nep Protein, Mouse; PTK3A Protein, Mouse

• Gene Name:

  Discoidin domain receptor tyrosine kinase 1

• UniProt:

  Q03146-1

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Receptor Tyrosine Kinases (RTKs)

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Kinase proteins, CD Molecule proteins, Enzyme Proteins

• Molecular Weight:

  The secreted recombinant mouse DDR1 comprises 406 amino acids and has a calculated molecular mass of 45.4 kDa. As a result of glycosylation, the apparent molecular mass of rm DDR1 is approximately 50 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  DDR1

• Species:

  Mouse

• Overview:

  A DNA sequence encoding the extracellular domain of mouse DDR1 (NP_766550.1) (Met 1-Thr 414) was expressed, with a C-terminal polyhistidine tag.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q03146-1

• AA Sequence:

  Met1-Thr414