Mouse DDR1 Protein, Fc Tag

Mouse DDR1 Protein, Fc Tag

• Background: Discoidin domain receptor 1 (DDR1) is a member of DDRs, which are members of the receptor tyrosine kinase (RTK). Upon collagen binding, DDR1 undergoes tyrosine autophosphorylation, which consequently triggers downstream genetic and cellular pathways and plays critical roles in the regulation of cellular morphogenesis, differentiation, proliferation, adhesion, migration, and invasion. Research shows that DDR1 is closely related to various human diseases including cancer, fibrosis, atherosclerosis, and other inflammatory disorders. New generation DDR1 inhibitors targeting the allosteric sites outside of the canonical ATP-binding pocket or extracellular domain (allosteric inhibitors) may offer a new opportunity for selective DDR1 inhibition therapy development.
• Description: Mouse DDR1, Fc Tag (DD1-M5259) is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Thr 414 (Accession # Q03146-1).
• Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 70.2 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Asp 22 - Thr 414
• Host: HEK 293
• Target: DDR1
• Conjugation: Unconjugated
• Tag: C-Fc
• Source: Mouse
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Native Human Collagen I protein at 10 μg/mL (100 μL/well) can bind Mouse DDR1, Fc Tag (Cat. No. DD1-M5259) with a linear range of 0.039-1.25 μg/mL (QC tested).
• Format: Powder
• Buffer: 50 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl,pH7.5
• Additives: Trehalose
• Molecular Weight: 70.2 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C