CD31 Protein, Mouse, Recombinant (His Tag)

• Background:

  The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

• Synonyms:

  C85791 Protein, Mouse; Cd31 Protein, Mouse; Pecam Protein, Mouse; PECAM-1 Protein, Mouse

• Gene Name:

  Platelet/endothelial cell adhesion molecule 1

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Hemangioblast Markers ITIM/ITAM Immunoreceptors and Related Molecules

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 93 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  CD Molecule proteins, Biomarker Proteins, Diagnostic proteins

• Molecular Weight:

  The secreted recombinant mouse PECAM1 consists of 584 amino acids and has a calculated molecular mass of 66 kDa. The apparent molecular mass of the recombinant protein is approximately 85-95 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PECAM1

• Species:

  Mouse

• Protein ID:

  NP_033868.3

• Overview:

  A DNA sequence encoding the mouse PECAM1 (NP_033868.3) extracellular domain (Met 1-Lys 590) was expressed, with a C-terminal polyhistidine tag.

• AA Sequence:

  Met1-Lys590

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_033868.3