SIRP alpha Protein, Human, Recombinant (ECD, SIRP alphaV8, His Tag)

• Background:

  Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

• Synonyms:

  BIT Protein, Human; CD172A Protein, Human; MFR Protein, Human; MYD-1 Protein, Human; P84 Protein, Human; PTPNS1 Protein, Human; SHPS1 Protein, Human; SIRP Protein, Human; SIRP alpha Protein, Human

• Gene Name:

  Signal-regulatory protein alpha

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured by its binding ability in a functional ELISA. Immobilized human CD47 (Cat: 12283-HCCH) at 2 μg/ml (100 μl/well) can bind human SIRPa (V8) -His (Cat: PH52574M2), the ECof human SIRPa (V8) -His is 150-500 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant human SIRP alphaV8 consists of 350 amino acids and predicts a molecular mass of 38.6 kDa. As a result of glycosylation, it migrates as an approximately 49.9 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SIRPA

• Species:

  Human

• Protein ID:

  AAH38510.1

• Overview:

  A DNA sequence encoding the human SIRP alphaV8 (AAH38510.1, with mutation Ser 44 Leu, Ser 50 Thr, Ile 52 Thr, His 54 Arg, Val 57 Ala) (Met1-Arg369) was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Arg369 (S44L, S50T, I52T, H54R, V57A)

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/AAH38510.1