CFHR1 Protein, Human, Recombinant (His Tag)

• Background:

  CFHR1 is a secreted protein belonging to the complement factor H protein family.The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (CFHR-1 to -5) . Members of the H-related protein family are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. CFHR1 binds to Pseudomonas aeruginosa elongation factor Tuf together with plasminogen, which is proteolytically activated. CFHR1 might be involved in complement regulation. It can associate with lipoproteins and may play a role in lipid metabolism.

• Synonyms:

  CFHL Protein, Human; CFHL1 Protein, Human; CFHL1P Protein, Human; CFHR1 Protein, Human; CFHR1P Protein, Human; FHR1 Protein, Human; H36-1 Protein, Human; H36-2 Protein, Human; HFL1 Protein, Human; HFL2 Protein, Human

• Gene Name:

  Complement factor H related 1

• UniProt:

  Q03591

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Serine Proteases and Regulators

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Biomarker Proteins

• Molecular Weight:

  The recombinant human CFHR1 consists of 323 amino acids and predicts a molecular mass of 37.2 KDa. It migrates as an approximately 45 KDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CFHR1

• Species:

  Human

• Overview:

  A DNA sequence encoding the human CFHR1 (Q03591) (Met1-Arg330) was expressed with a polyhistidine tag at the C-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q03591

• AA Sequence:

  Met1-Arg330