Aminopeptidase A Protein, Human, Recombinant (His Tag)

• Background:

  ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.

• Synonyms:

  APA Protein, Human; CD249 Protein, Human; ENPEP Protein, Human; gp160 Protein, Human

• Gene Name:

  Glutamyl aminopeptidase

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin (Glu-AMC) . The specific activity is >2000 pmoles/min/μg.

• Form:

  Liquid

• Buffer:

  Supplied as sterile 20 mM, Tris 500 mM NaCl, 10 % glycerol, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins, CD Molecule proteins

• Molecular Weight:

  The recombinant human ENPEP consists of 933 amino acids and has a calculated molecular mass of 107.2 kDa. The recombinant protein migrates as an approximately 115.5 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  Liquid. It is shipped out with blue ice. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ENPEP

• Species:

  Human

• Protein ID:

  NP_001968.3

• Overview:

  A DNA sequence encoding the human ENPEP (NP_001968.3) (Arg41-Gly957) was expressed with a polyhistide tag at the N-terminus.

• AA Sequence:

  Arg41-Gly957

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_001968.3