CCL5/RANTES Protein, Human, Recombinant (His & mucin Tag)

• Background:

  Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokin ligand 5 (CCL5) is chemotactic for T cells, basophils and eosinophils. Chemokin ligand 5 (CCL5) has been considered a HIV-supressor secreted by CD8T cells and other immune cells. Chemokin ligand 5 (CCL5) is a key to activating recruit leukocytes into inflammatory sites and in the presence of particular cytokines released by T cells, it can change the NK cells into CHAK cells.

• Synonyms:

  D17S136E Protein, Human; eoCP Protein, Human; RANTES Protein, Human; SCYA5 Protein, Human; SIS-delta Protein, Human; SISd Protein, Human; TCP228 Protein, Human

• Gene Name:

  Chemokine (C-C motif) ligand 5

• Expression System:

  HEK293 Cells

• Tag:

  C-Mucin & His

• Field of Research:

  Neuroinflammation

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Immunotherapy proteins, Cytokine Proteins, Growth Factor proteins, Biological Target proteins, Biomarker Proteins

• Molecular Weight:

  The recombinant human RANTES/Mucin stalk chimera consists of 318 amino acids and has a predicted molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh RANTES/Mucin stalk chimera is approximately 50-80 kDa due to glycosylation.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CCL5

• Species:

  Human

• Protein ID:

  NP_002976.2

• Overview:

  A DNA sequence encoding the human RANTES (NP_002976.2) (Met 1-Ser 91) was fused with the C-terminal polyhistidine-tagged human Fractalkine mucin-stalk (Phe 101-Gln 341) at the C-terminus.

• AA Sequence:

  Met1-Ser91 & Phe101-Gln341

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_002976.2