NRG1 Alpha Protein, Human, Recombinant (ECD, His Tag)

• Background:

  Neuregulin 1 or NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. This growth factor was originally identified as a 44-kD glycoprotein that interacts with the NEU / ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. NRG1 is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by the usage of different promoters and alternative splicing of a single gene. Neuregulin 1 (NRG1) is essential for the development and function of multiple organ systems, and its dysregulation has been linked to diseases such as cancer and schizophrenia. NRG1 is a schizophrenia candidate gene and plays an important role in brain development and neural function. Schizophrenia is a complex disorder, with etiology likely due to epistasis.

• Synonyms:

  ARIA Protein, Human; GGF Protein, Human; GGF2 Protein, Human; HGL Protein, Human; HRG Protein, Human; HRG1 Protein, Human; HRGA Protein, Human; MST131 Protein, Human; MSTP131 Protein, Human; NDF Protein, Human; Neuregulin 1 Protein, Human; NRG1-IT2 Protein, Human; SMDF Protein, Human

• Gene Name:

  Neuregulin 1

• UniProt:

  Q02297-1

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by SDS-PAGE.

• Activity:

  1. Measured by its ability to biotinylated Human ErbB4-Fc (cat:10363-H03H) in functional Elisa. 2. Measured by its ability to biotinylated Rhesus ErbB3-His (Cat:PK54066M2) in functional Elisa. 3. Measured by its ability to biotinylated Rhesus ErbB3 (Cat:PK54182M4) in functional Elisa.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Growth Factor proteins, Biological Target proteins, Cytokine Proteins, epigenetic proteins, Enzyme Proteins

• Molecular Weight:

  The secreted recombinant human NRG1 consists of 233 amino acids and has a calculated molecular mass of 25.5kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NRG1 is approximately 40-45 kDa due to glycosylation.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  NRG1

• Species:

  Human

• Overview:

  A DNA sequence encoding the human NRG1 isoform alpha (Q02297-1) extracellular domain (Ser 20-Lys 241) was fused with a polyhistidine tag at the C-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q02297-1

• AA Sequence:

  Ser20-Lys241