Mark3 Protein, Human, Recombinant (His & GST Tag)

• Background:

  MAP/microtubule affinity-regulating kinase 3, also known as C-TAK1, cTAK1, Cdc25C-associated protein kinase 1, ELKL motif kinase 2, Protein kinase STK1, Ser/Thr protein kinase PAR-1, Serine/threonine-protein kinase p78, MARK3, CTAK1, and EMK2, is a ubiquitously expressed protein which belongs to the protein kinase superfamily, CAMK Ser / Thr protein kinase family and MARK subfamily. MARK3 contains one KA1 (kinase-associated) domain, one protein kinase domain, and one UBA domain. The Par-1 / MARK protein kinases play a pivotal role in establishing cellular polarity. This family of kinases contains a unique domain architecture, in which a ubiquitin-associated (UBA) domain is located C-terminal to the kinase domain. MARKs / PAR-1 are common regulators of cell polarity that are conserved from nematode to human. All of these kinases have a highly conserved C-terminal domain, which is termed the kinase-associated domain 1 (KA1) .

• Synonyms:

  CTAK1 Protein, Human; KP78 Protein, Human; Par-1a Protein, Human; PAR1A Protein, Human

• Gene Name:

  MAP/microtubule affinity-regulating kinase 3

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-GST & His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 88 % as determined by SDS-PAGE.

• Activity:

  The specific activity was determined to be 7 nmol/min/mg using synthetic CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.

• Form:

  Liquid

• Buffer:

  Supplied as sterile 20mM Tris, 500mM NaCl, 0.25mM DTT, 0.1mM PMSF, 0.1mM EDTA, pH 7.4, 10% gly Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Kinase proteins, Enzyme Proteins

• Molecular Weight:

  The recombinant human MARK3/GST chimera consists of 967 amino acids and has a calculated molecular mass of 109.3 kDa. It migrates as an approximately 120 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  MARK3

• Species:

  Human

• Protein ID:

  AAH24773.1

• Overview:

  A DNA sequence encoding full length of human MARK3 (AAH24773.1) (Met 1-Leu 729) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

• AA Sequence:

  Met1-Leu729

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/AAH24773.1