SIGLEC5 Protein, Human, Recombinant (hFc Tag)

• Background:

  SIGLEC5 contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. It belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. SIGLEC5 is expressed by monocytic/myeloid lineage cells. It is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is also expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

• Synonyms:

  CD170 Protein, Human; CD33L2 Protein, Human; OB-BP2 Protein, Human; OBBP Protein, Human; OBBP2 Protein, Human; SIGLEC-5 Protein, Human; SIGLEC5 Protein, Human

• Gene Name:

  Sialic acid binding Ig like lectin 5

• UniProt:

  O15389

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Field of Research:

  ITIM/ITAM Immunoreceptors and Related Molecules

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Immune Checkpoint Proteins, Biological Target proteins, CD Molecule proteins

• Molecular Weight:

  The recombinant human SIGLEC5 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 659 amino acids and has a predicted molecular mass of 73.4 kDa. The apparent molecular mass of the protein is approximately 74-93 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SIGLEC5

• Species:

  Human

• Overview:

  A DNA sequence encoding the human SIGLEC5 (O15389) (Met1-Thr 434) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/O15389

• AA Sequence:

  Met1-Thr434