CD47 Protein, Human, Recombinant (ECD, hFc Tag)

• Background:

  CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.

• Synonyms:

  IAP Protein, Human; MER6 Protein, Human; OA3 Protein, Human

• Gene Name:

  CD47 molecule

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  1. Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His (Cat:PH50493M2) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The ECof human CD47-Fc is 10.1-23.5 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized mouse SIRPA-His (Cat:PM52920M2) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The ECof human CD47-Fc is 0.05-0.13 μg/ml. 3. Measured by its binding ability in a functional ELISA. Immobilized human SIRPG-His (Cat:PH52563M2) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The ECof human CD47-Fc is 0.58-1.34 μg/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Immune Checkpoint Proteins, Biological Target proteins, CD Molecule proteins, Cancer proteins, Immunotherapy proteins

• Molecular Weight:

  The recombinant human CD47/Fc is a disulfide-linked homodimer. The reduced monomer comprises 362 amino acids and has a predicted molecular mass of 40.7 kDa. The apparent molecular mass of the protein is approximately 48-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CD47

• Species:

  Human

• Protein ID:

  NP_942088.1

• Overview:

  A DNA sequence encoding the human CD47 (NP_942088.1) (Met1-Pro139) was expressed with the Fc region of human IgG1 at the C-terminus.

• AA Sequence:

  Met1-Pro139

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_942088.1