LOXL2 Protein, Human, Recombinant (His Tag)

• Background:

  Lysyl oxidase homolog 2, also known as Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14 and LOXL2, is a secreted protein that belongs to the lysyl oxidase family. LOXL2 contains four SRCR domains. The lysyl oxidase family is made up of five members: lysyl oxidase (LOX) and lysyl oxidase-like 1-4 (LOXL1, LOXL2, LOXL3, LOXL4) . All members share conserved C-terminal catalytic domains that provide for lysyl oxidase or lysyl oxidase-like enzyme activity; and more divergent propeptide regions. LOX family enzyme activities catalyze the final enzymatic conversion required for the formation of normal biosynthetic collagen and elastin cross-links. LOXL2 is expressed by pre-hypertrophic and hypertrophic chondrocytes in vivo, and that LOXL2 expression is regulated in vitro as a function of chondrocyte differentiation. LOXL2 promotes chondrocyte differentiation by mechanisms that are likely to include roles as both a regulator and an effector of chondrocyte differentiation. LOXL2 expression could also be explored as a molecular target in the prevention of breast cancer progression.

• Synonyms:

  LOR2 Protein, Human; Lysyl oxidase-like 2 Protein, Human; WS9-14 Protein, Human

• Gene Name:

  Lysyl oxidase-like 2

• UniProt:

  Q9Y4K0

• Expression System:

  CHO Stable Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine.The specific activity is >2pmol/min/μg

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20 mM MES, 50 mM NaCl Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins, Protease proteins

• Molecular Weight:

  The recombinant human LOXL2 consists of 760 amino acids and has a predicted molecular mass of 85.5 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  LOXL2

• Species:

  Human

• Overview:

  A DNA sequence encoding the human LOXL2 (Q9Y4K0) (Met1-Gln774) was expressed, fused with a polyhistidine tag at the C-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9Y4K0

• AA Sequence:

  Met1-Gln774