IL-7 Protein, Human, Recombinant

• Background:

  IL7, also known as interleukin 7, is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. IL7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V (D) J rearrangement of the T cell receptor beta (TCRβ) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.

• Synonyms:

  IL-7 Protein, Human; Interleukin-7 Protein, Human

• Gene Name:

  Interleukin 7

• Expression System:

  E. coli

• Tag:

  No tag

• Endotoxin:

  < 0.01 EU per μg of the protein.

• Purity:

  ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

• Activity:

  Measured in a cell proliferation assay using anti-CD3 antibody activated human peripheral blood mononuclear cell (PBMC) . The EDfor this effect is typically 0.5-8 ng/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Growth Factor proteins, Interleukin proteins, Immunotherapy proteins, Biological Target proteins, Cell Therapy proteins

• Molecular Weight:

  The recombinant human IL7 consists of 153 amino acids and predicts a molecular mass of 17.5 KDa. It migrates as an approximately 16.44 KDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IL7

• Species:

  Human

• Protein ID:

  NP_000871.1

• Overview:

  A DNA sequence encoding the mature form of human IL7 (NP_000871.1) (Asp26-His177) was expressed with a N-terminal Met.

• AA Sequence:

  Asp26-His177

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_000871.1