S100A1 Protein, Human, Recombinant

• Background:

  S100A1 is a Cabinding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. This protein has been shown to function in the processes including stimulation of Ca-induced Carelease, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.

• Synonyms:

  S100 Protein, Human; S100-alpha Protein, Human; S100A Protein, Human

• Gene Name:

  S100 calcium binding protein A1

• Expression System:

  E. coli

• Tag:

  No tag

• Field of Research:

  Calcium-binding Proteins and Related Molecules

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 97 % as determined by SDS-PAGE.

• Activity:

  1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant human Fc-S100B (Cat:PH50959M3) at 10 μg/mL (100 μl/well) can bind biotinylated human S100A1 (Cat:PH51054E0) with a linear range of 15.6-250 ng/mL. 3. Measured by its ability to bind human His-S100B (Cat:PH52388E1) in functional ELISA.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Biomarker Proteins, Diagnostic proteins

• Molecular Weight:

  The recombinant human S100A1 consists of 94 amino acids and has a predicted molecular mass of 10.5 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  S100A1

• Species:

  Human

• Protein ID:

  NP_006262.1

• Overview:

  A DNA sequence encoding the human S100A1 (NP_006262.1) (Met 1-Ser 94) was expressed.

• AA Sequence:

  Met1-Ser94

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_006262.1