Her2/ERBB2 Protein, Human, Recombinant (aa 676-1255, His & GST Tag)

• Background:

  Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

• Synonyms:

  CD340 Protein, Human; ENV Protein, Human; ENVW Protein, Human; ERVWE1 Protein, Human; HER-2 Protein, Human; HER-2/neu Protein, Human; HER2 Protein, Human; HERV-7q Protein, Human; HERV-W-ENV Protein, Human; HERV7Q Protein, Human; HERVW Protein, Human; HERVWENV Protein, Human; MLN 19 Protein, Human; MLN19 Protein, Human; NEU Protein, Human; NGL Protein, Human; TKR1 Protein, Human

• Gene Name:

  Erb-b2 receptor tyrosine kinase 2

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-GST & His

• Field of Research:

  Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  No Kinase Activity

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 8.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Kinase proteins, Growth Factor proteins, CD Molecule proteins, CAR T Cell Therapy Target Proteins, Receptor Proteins, Cancer proteins, Immunotherapy proteins, Biomarker Proteins, Cytokine Proteins, Enzyme Proteins, Biological Target proteins, Diagnostic proteins

• Molecular Weight:

  The recombinant human ErbB2 (676-1255) /GST chimera consists of 817 amino acids and predictes a molecular mass of 92 kDa. It migrates as an approximately 100 KDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ERBB2

• Species:

  Human

• Protein ID:

  NP_004439.2

• Overview:

  A DNA sequence encoding the cytoplasmic domain (Lys 676-Val 1255) of human ErbB2 (NP_004439.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

• AA Sequence:

  Lys676-Val1255

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_004439.2